2 credits
Spring 2026 Lecture
This course provides life science graduate students with fundamental concepts and knowledge related to how modern light microscopes can be used to observe, measure, and manipulate biological samples. Completion of this course will help students understand the capabilities and limitations of current technologies. This knowledge can be used to design new experiments that successfully incorporate modern imaging technologies.
Learning Outcomes1Understand ray and wave properties of light and how they affect image formation in a microscope.
2Understand the different optical components of a light microscope and how they affect the light to form an image.
3Understand the principles of modern imaging techniques with a focus on fluorescence to be able to incorporate them into their own research program.
4Know the dos and don'ts in sample preparation and imaging.
5Know the potential and limitations of digital image processing and analysis.
Restrictions
made by Purdue students.