3 credits
Spring 2026 Lecture
The purpose of this course is to give graduate students an overview of proteomics and metabolomics research with an emphasis on mass spectrometric technologies and biological applications. Topics will include mass spectrometric instrumentation, protein peptide, and metabolite separation methods, methods for selective enrichment of modified proteins and peptides, identification of proteins and post-translational modifications, metabolite identification and quantification, targeted and untargeted analysis, and pathway mapping. We will also discuss examples of omics studies and the impact they are having on biological and biomedical research. A major focus will be training students to analyze and interpret data. This course is intended for graduate students interested in using mass spec approaches in their own research or simply learning about this technology.
Learning Outcomes1Be knowledgeable in practical skills associated with mass spectral analysis of biological molecules, with a heavy emphasis on proteins.
2Understand the basics of experimental design for the mass spectral analysis of proteins and metabolites.
3Acquire an appreciation for current instrumentation and methods commonly used in biological mass spectrometry and the advantages and disadvantages of each.
4Read and critically evaluate primary literature pertaining to proteomic and metabolomic studies.
Restrictions
made by Purdue students.