0 or 2 credits
Fall 2024 Lecture Laboratory Upper Division
This course aims to provide students an introduction to the theory and practice of functional genomics in plants. The course is centered around the many forms in which DNA sequencing technologies are applied to understand plant genomes, their variation and their function. The course will cover concepts in plant genome sequencing, transcriptome profiling and functional enrichment analyses in sets of genes. This is a full semester course that meets twice a week for: 1. A lecture that covers the rationale and theory of the topic and 2. A hands-on lab section that provides experiential learning with the computational tools and packages used in plant genomics and transcriptomics.
Learning Outcomes1Describe and compare the chemistry, data yield, and cost of first generation, next generation and third generation sequencing technologies, and their applications in plant genome sequencing.
2Obtain and understand the information on genome sequences and gene annotations for a sequenced plant genome.
3Design and perform a transcriptome analysis pipeline.
4Describe the statistical basis of gene enrichment analysis.
5Apply standard gene group analysis tools to perform GO term analysis, motif analysis, pathway analysis, gene list overlapping and gene regulatory network analysis to interpret a gene list resulting from a functional genomics project.
Restrictions
made by Purdue students.