0 or 2 credits
Fall 2025 Laboratory Lower DivisionScience
(IT 22600) Focuses on nucleic acid manipulation. Modules include, making a eukaryotic library, identifying clones, sub-cloning into a bacterial expression vector and verification of the clone's identity by restriction analysis and DNA sequencing. Basic laboratory techniques (solution making, buffer preparation, good safety techniques), sterile technique and compliance procedures. ABE 22600 content is primarily based on biological sciences, not engineering.
Learning Outcomes1Perform techniques currently used in cell, molecular, and microbiology, while understanding the rationale behind the specific approaches.
2Explain the experimental basis of techniques used, indicating the significance of the work, presenting, calculating, and discussing the data, and drawing conclusions.
3Given a specific biological question, the student will be able to determine appropriate applications of specific cell, molecular, and microbiological techniques.
4Gain experience in dissecting and extracting pertinent information from scientific journal articles.
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