GPA

3.29

RedditCatalog

Modern biochemical techniques for the purification and characterization of biological proteins. This is a project-oriented course where students begin by purifying a recombinant enzyme by affinity chromatography and then characterize various biochemical properties of the enzyme throughout the semester. Emphasis will be placed on quantitative analyses, including measurements of enzyme activity and inhibition, molecular interactions, and oligomeric state. Students will learn basic principles of designing assays to measure biochemical phenomena. Use of bioinformatics and computational modeling tools for protein structure analysis will be integrated. The course will culminate with preparation of a manuscript-style report describing the enzyme characterization.

Prerequisites

Attachments